[ Instrument Network Instrument Development ] Recently, the intelligent research institute of the intelligent micro-nano device research institute has made new progress in the visual analysis and detection of environmental hazards. The team designed and prepared a high-efficiency colorimetric fluorescent nanoprobe, which was printed into a fluorescent test paper. According to the color display of the test paper, the detection of lead ions in water can be preliminarily determined, and the on-site visualization and semi-quantitative determination of lead ions in water can be realized. Detection. Related results have been published in the analytical field TOP journal Analytical Chemistry.
Figure: a) Visualization of lead ions in water; b) Ratio of fluorescent probes to detect the selectivity of lead ions; c) Ratio of fluorescent probes to detect the selectivity of lead ions corresponding to fluorescent images (photographed under 365 nm UV light); d) A schematic diagram of the detection of lead ions by a smartphone; e) a linear relationship between the B/R value of the smartphone and the concentration of lead ions.
Because lead ions greatly damage human health and pollute the environment, it is considered to be one of the most toxic heavy metal ions. Traditional lead ion detection methods, including stripping voltammetry (SV), atomic absorption spectroscopy (AAS), atomic emission spectroscopy (AES), and inductively coupled plasma mass spectrometry (ICP-MS), can achieve sensitivity to lead ions. And selective analysis, but its expensive instrumentation, long inspection cycle and the need for professional operations limit its application in field detection.
Researchers at the Institute of Intelligent Research have developed a method for detecting lead ions in water using a ratiometric fluorescent probe, and the fluorescent test paper printed with the fluorescent probe solution can be used for detection. The ratio of the fluorescent probe is obtained by mixing blue and red carbon dots in a proper ratio. When the lead ions are present, the blue carbon dot fluorescence is quenched and the red carbon dot fluorescence is unchanged, so that an obvious observation is observed under ultraviolet light irradiation. Blue to red color changes. Visualization, real-time and semi-quantitative detection of lead ions can be achieved by identifying the smartphone APP (color recognizer) or other color recognition card by color RGB values â€‹â€‹of the test strip.
This ratio probe is prepared with low toxicity carbon dots and is environmentally friendly. The detection limits of fluorescent probe solutions using fluorescence spectrometer and smartphone APP are 2.89nM and 35.26nM, respectively, which are far lower than lead ions in WHO drinking water. Allowable limit (10mgÂ·L-1). Using the smart phone to identify the RGB value of the fluorescent paper strip, the on-site, fast and semi-quantitative detection of lead ions in the water can be completed in 5 minutes. Fluorescent paper strips are easy to store and carry, making the method of detecting lead ions more convenient, simple, time-saving and cost-effective.
This work is the team's further deepening based on the fluorescence analysis test paper research. The series also featured Fluorescent Nanomaterials for Color-Multiplexing Test Papers toward Qualitative/Quantitative Assays and was published in the Small Method in the form of a guest review article.
The research work was supported by the National Natural Science Foundation of China, the National 973 Major Research Project, and the Key Research and Development Program of Anhui Province.
(Original title: On-site visualization and semi-quantitative detection of lead ions in water realized by intelligence)
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