Electrospore catcher captures the incidence of ascospores in wheat
2021-11-03 00:05:32
Predicting the occurrence of wheat scab in the past was mainly based on the use of meteorological factors, but this method alone may not be sufficient. In order to improve the accuracy of the prediction of the occurrence of wheat scab, the pathogenic bacteria are mainly scattered on the ascendant, and the sample is captured using an electrospore catcher. The results show that the accuracy of the prediction method using the meteorological factor is higher than before.
The capture of the ascus capsules was carried out using an agarose spore catcher to investigate the scattering of the ascus capsules on a daily basis. The electrospore catcher is located 30 centimeters high near the center of the field. Apply a mixture of well-adjusted glycerin to the glass slide. Place an agarose trap on top and inside. Replace each day. Check the number of spheroids in a 18x18 mm cover slip with a microscope at 100^r400. . The incidence of wheat scab and the hazard rate in the field of measurement were investigated for 4 varieties (2 rye and 2 wheat), and 100 spikes for each variety were examined.
The number of scabs in different years of the scab fungus was generally captured in the early April and the wheat heading in mid-April. However, in the few years of catch, the middle and the middle of April Can not catch robes. In addition, the captured sporangium is considered to be the ascospore spore of Fusarium head blight, and it may also be the gauze gown of another species of gibberellica. Therefore, it is always referred to as gibberella. The relationship between the number of ascus capsules captured and the onset: the hazard rate of the measurement and reporting field, the occurrence of prevention and control of the counties in Tianhe County and Ehime County, and almost no correlation with the number of spores in early April; the number of spores since mid-April The correlation becomes higher gradually, and it can be thought that this time is the main sensible period; the correlation with the upper sentence from the end of April to May is the highest.
Analysis of the samples captured by the electrospore catcher revealed that the number of blisters in April can predict the occurrence of Gibberella mori. However, when the local government decides whether or not to use chemical control, the prediction using the number of spores in mid-April is considered to be the most appropriate, although the accuracy is slightly lower than predicted by the number of spores in April. This method of predicting the number of spores is based on the results of the study focused on pathogenic bacteria. It is of course important to attribute the main cause of the disease to pathogens. However, it is also important to consider whether or not the onset period coincides with the heading and flowering period of crops, and the environmental conditions of the onset of pathogenicity, such as meteorological conditions (especially rainfall), are studied together.
The capture of the ascus capsules was carried out using an agarose spore catcher to investigate the scattering of the ascus capsules on a daily basis. The electrospore catcher is located 30 centimeters high near the center of the field. Apply a mixture of well-adjusted glycerin to the glass slide. Place an agarose trap on top and inside. Replace each day. Check the number of spheroids in a 18x18 mm cover slip with a microscope at 100^r400. . The incidence of wheat scab and the hazard rate in the field of measurement were investigated for 4 varieties (2 rye and 2 wheat), and 100 spikes for each variety were examined.
The number of scabs in different years of the scab fungus was generally captured in the early April and the wheat heading in mid-April. However, in the few years of catch, the middle and the middle of April Can not catch robes. In addition, the captured sporangium is considered to be the ascospore spore of Fusarium head blight, and it may also be the gauze gown of another species of gibberellica. Therefore, it is always referred to as gibberella. The relationship between the number of ascus capsules captured and the onset: the hazard rate of the measurement and reporting field, the occurrence of prevention and control of the counties in Tianhe County and Ehime County, and almost no correlation with the number of spores in early April; the number of spores since mid-April The correlation becomes higher gradually, and it can be thought that this time is the main sensible period; the correlation with the upper sentence from the end of April to May is the highest.
Analysis of the samples captured by the electrospore catcher revealed that the number of blisters in April can predict the occurrence of Gibberella mori. However, when the local government decides whether or not to use chemical control, the prediction using the number of spores in mid-April is considered to be the most appropriate, although the accuracy is slightly lower than predicted by the number of spores in April. This method of predicting the number of spores is based on the results of the study focused on pathogenic bacteria. It is of course important to attribute the main cause of the disease to pathogens. However, it is also important to consider whether or not the onset period coincides with the heading and flowering period of crops, and the environmental conditions of the onset of pathogenicity, such as meteorological conditions (especially rainfall), are studied together.
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